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Monday, 2 June 2014

PREPARATION OF 4% BORIC ACID WITH SCREENED METHYL RED

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PREPARATION OF 4% BORIC ACID WITH SCREENED METHYL RED

This is a reagent used in the analysis of protein in the Kjedahl method for collecting the condensed ammonia during distillation.

It is a useful weak acid for analysis of organic substances

Procedure

The procedure to prepare 1000ml 4% Boric acid with screened methyl red is as follows:

APPARATUS: 500ml Beaker, stirrer, 1000ml volumetric flask

REAGENT: Deionized water, Boric acid powder, Bromo cresol green, methyl red

PROCEDURE:

1.      Weigh 40g of Boric acid (B(OH)3) powder into a 500ml beaker, add 200ml deionized water and stir to dissolve

2.      Transfer to 1000ml volumetric flask,

3.      Add 10ml Bromocresol green, 7ml Methyl red, and make up to the mark with deionized water and shake.

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