PREPARATION OF 4% BORIC ACID
WITH SCREENED METHYL RED
This is a reagent used in the analysis of protein in
the Kjedahl method for collecting the condensed ammonia during distillation.
It is a useful weak acid for analysis of organic
substances
Procedure
The procedure to prepare 1000ml 4%
Boric acid with screened methyl red is as follows:
APPARATUS:
500ml Beaker, stirrer, 1000ml volumetric flask
REAGENT:
Deionized water, Boric acid powder, Bromo cresol green, methyl red
PROCEDURE:
1. Weigh
40g of Boric acid (B(OH)3) powder into a 500ml beaker, add 200ml deionized
water and stir to dissolve
2. Transfer
to 1000ml volumetric flask,
3. Add
10ml Bromocresol green, 7ml Methyl red, and make up to the mark
with deionized water and shake.
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DeleteCan we use boric acid immediately after preparing for nitrogen analysis or we have to wait for 1 day before using?
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