Preparation of 0.2N Phosphate buffer of pH 5.8

Preparation of 0.2N Phosphate buffer of pH 5.8

1.      Weigh 54.44g of potassium dihdro-orthophosphate (KH₂PO₄) into a beaker

2.      Transfer and washed into a 2000ml volumetric flask with deionized/distilled water

3.      Make up to the mark with deionized/distilled water.

4.      Measure 1250ml of the solution into 5000ml beaker to read the pH.

5.      The pH value should be measured with a pH meter and the pH should be in the region of 4.21

6.      Increase to 5.8 with 0.2M NaOH in drops with continuous stirring.

7.      Transferred the solution into a 5 litre volumetric flask

8.      Make up to the mark with deionized/distilled water (pH 7).