Preparation of 0.5M Acetate buffer

Preparation of 0.5M Acetate buffer

1.      Weigh 2.99g of sodium acetate trihydrate into a beaker

2.      Transferred into 1000ml volumetric flask with half filled with distilled water

3.      Add 1.66ml of glacial acetic acid

4.      Make up to the mark with distilled water.

5.      The pH of the solution should be measured with a pH meter and should be 4.51  at 37C.