SODIUM MONOGLUTAMATE TEST
AIM: To determine the amount of glutamate in seasoning
APPARATUS: 250ml conical flask, measuring cylinder, 50ml Beaker.
REAGENT: 0.1M NaOH, Bromothymol blue
PRINCIPLE: This is a neutralization reaction between the acid (glutamate) and base (NaOH)
PROCEDURE:
Weigh 0.5g of the sample ( in granulated/powder) in a 250ml conical flask
Add 250ml of distilled water using measuring cylinder and heat to boil, then allow to cool
Add 2 drops of Bromothymol blue and titrate against 0.1M NaOH In the burette to a deep blue colour end point is obtained
Calculation
% Glutamate = Titre value * 14.7 * 100%
Weight taken
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