Phosphate Buffer (pH 7.4)
Purpose: Used in biological experiments to maintain pH close to physiological conditions.
Procedure:
Dissolve 8.0 g NaCl, 0.2 g KCl, 1.44 g Na₂HPO₄, and 0.24 g KH₂PO₄ in 800 ml distilled water.
Adjust pH to 7.4 using HCl or NaOH.
Make up to 1000 ml with distilled water.
Store at 4°C if not used immediately.
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IKAWO OLORUNWA EMMANUEL is the CED @ ACTIONASE POINT. He is a Researcher, Biochemist/Pharmacologist, Author, Self Help Author, Motivational Speaker, Home Business Trainer, Marketing, Sales and Management Expert. This blog was inspired by His Internship with National Agency for Food and Drugs Administration and Control (NAFDAC) and His love for scientific data sharing.
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