How to Prepare Biuret Reagent - BIOSCIENCE EDUCATION

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Friday, 7 November 2025

How to Prepare Biuret Reagent

Biuret Reagent


Purpose: Detects proteins (peptide bonds).

Procedure:

  1. Dissolve 1.5 g CuSO₄·5H₂O in 500 ml distilled water.

  2. Add 6 g sodium potassium tartrate and 375 ml of 10% NaOH.

  3. Make up to 1000 ml with distilled water.

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