DETERMINATION OF CHLOROPHYLL IN LEAF EXTRACT
AIM:
To estimate chlorophyll content in a leaf sample.
APPARATUS:
Spectrophotometer, centrifuge, test tubes.
REAGENT:
80% acetone.
PRINCIPLE:
Chlorophyll is extracted and measured at specific absorbance values.
PROCEDURE:
Grind 1g of leaf in 10ml acetone.
Centrifuge and collect supernatant.
Measure absorbance at 645 nm and 663 nm.
CALCULATION:
Use Arnon's formula:
Chlorophyll a = 12.7(A663) – 2.69(A645)
Chlorophyll b = 22.9(A645) – 4.68(A663)
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