HOW TO DETERMINE CITRIC ACID IN FRUIT JUICE - BIOSCIENCE EDUCATION

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Monday, 26 January 2026

HOW TO DETERMINE CITRIC ACID IN FRUIT JUICE

DETERMINATION OF CITRIC ACID IN FRUIT JUICE

AIM:
To estimate citric acid content in fruit juice.

APPARATUS:
Burette, conical flask, pipette.

REAGENT:
0.1M NaOH, phenolphthalein.

PRINCIPLE:
Citric acid neutralized by NaOH; endpoint indicated by pink color.

PROCEDURE:

  • Pipette 10ml of juice into a flask.

  • Add 2–3 drops phenolphthalein.

  • Titrate with NaOH to pink endpoint.

CALCULATION:
% Citric Acid = Titre × 0.064 × 100 / Volume of sample

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