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Friday, 20 June 2014

Preparation of Simulated Gastric Fluid (SGF)

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Preparation of Simulated Gastric Fluid (SGF)


Simulated Gastric Fluid (SGF) is a synthetic form of the gastric fluid in the stomach. This fluid will show the effect of the gastric juice in the stomach on a particular drug in the stomach.

Drugs made for dissolution and disintegration in the small intestine must not disintegrate or dissolve in the in the Simulated Gastric Fluid (SGF). On the other hand, drugs designed to act in the stomach will dissolve in this synthetic solution of Simulated Gastric Fluid (SGF).



To prepare Simulated Gastric Fluid (SGF)

1.      Weigh 2g of NaCl and 3.2g of Pepsin derived from stomach mucosa with an activity of about

2.      800 to 1200 units per milligram

3.      Add 7ml concentrated HCl to dissolve

4.      Transfer into 1000ml volumetric flask

5.      Add deionized/distilled water into 1000ml volumetric flask in drops

6.      Make up to the mark with deionized/distilled water.

7.      Test the pH with a pH meter and the pH should be a pH of 1.199.

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