CALCIUM TEST IN MILK (EDTA TITRATION)

CALCIUM TEST IN MILK (EDTA TITRATION)

AIM:
To estimate calcium content in milk.

APPARATUS:
Burette, pipette, conical flask.

REAGENT:
0.01M EDTA, buffer solution (pH 10), Eriochrome Black T.

PRINCIPLE:
EDTA binds calcium ions in presence of indicator, forming a color change at endpoint.

PROCEDURE:

• Pipette 10ml of milk (diluted), add buffer and indicator.

• Titrate with EDTA until the wine-red color changes to blue.

CALCULATION:
mg Ca²⁺ = Titre value × 40.08 (molar mass of Ca)