Friday, 31 October 2025

How to Prepare Barfoed’s Reagent

Barfoed’s Reagent


Purpose: Differentiates between monosaccharides and disaccharides.

Procedure:

  1. Dissolve 66.5 g copper(II) acetate in 1000 ml distilled water.

  2. Add 9 ml glacial acetic acid.

  3. Mix thoroughly and store in a dark bottle.

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