Friday, 17 October 2025

How to Prepare Benedict’s Reagent

Benedict’s Reagent

Purpose: Tests for presence of reducing sugars.

Procedure:

  1. Dissolve 173 g sodium citrate and 100 g anhydrous sodium carbonate in 800 ml distilled water.

  2. Separately dissolve 17.3 g CuSO₄·5H₂O in 100 ml water.

  3. Slowly add the CuSO₄ solution to the carbonate/citrate solution with constant stirring.

  4. Make up the volume to 1000 ml.

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