AD (728x90)

Powered by Blogger.

Friday, 9 May 2014

Preparation Of 0.05M Phosphate buffer of pH 6.8

Share it Please

Preparation Of 0.05M Phosphate buffer of pH 6.8

1.      Weigh 13.609g of monobasic potassium dihdro-orthophosphate (KH2PO4) into a beaker

2.      Transfer and washed into a 2000ml volumetric flask with deionized/distilled water

3.      Make up to the mark with deionized/distilled water.

4.      Measure 1250ml of the solution into 5000ml beaker to read the pH.

5.      The pH value should be 4.44 and increased to pH 6.8 with 0.2M NaOH in drops with continuous stirring.

6.      Transfer the solution into a 5 litre volumetric flask and made up to the mark with deionized/distilled water (pH 7).

Written by

We are Creative Blogger Theme Wavers which provides user friendly, effective and easy to use themes. Each support has free and providing HD support screen casting.

1 comments:

  1. Great blog created by you. I read your blog, its best and useful information. You have done a great work. Super blogging and keep it up.
    php jobs in hyderabad.

    ReplyDelete

© 2014 BIOSCIENCE EDUCATION. All rights resevered. No part of this blog can be reproduced without Reference. Designed by IKAWO O. E.