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Friday, 27 June 2014

Preparation of Simulated Intestinal Fluid (SIF)

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Preparation of Simulated Intestinal Fluid (SIF)

Simulated Intestinal Fluid is a synthetic fluid use in analysis of pharmaceuticals in the laboratory. The extent to which the analysed pharmaceutical dissolve and release it actives in the simulated intestinal is a good indicator of how the pharmaceutical will be available for absorption into the system from the villi in the intestine. The Simulated Intestinal Fluid is use to determine bioavailabilty of a particular drug when taken.


1.      Weigh 6.8g of monobasic potassium phosphate into a 500ml beaker

2.      Add 250ml of distilled water and mixed to dissolve.

3.      Add 77ml of 0.2N NaOH.

4.      Transfer the solution into 1000ml volumetric flask

5.      Add 300ml of water

6.      Add 1g of Pancreatine to the solution.

7.      Measure the pH (The pH will be in region of 4.5-4.6 at 25oC)

8.      Correct the pH of the solution to pH 6 by adding 0.2N NaOH to increase the pH value to 6.0 (0.2N HCl can also be used if the solution is basic).

9.      Make up the solution to the 1000ml mark.

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