PRAPARATION
OF FEHLING’S SOLUTIONS
Fehling solutions are vital reagent for Biochemist
and Chemists for qualitative and quantitative analysis of carbohydrates in the
laboratory. It was first prepared by Hermann
Von Fehling.
The following are the standard procedure for the
preparation of Fehling I and II solutions.
Preparation of Fehling I solution
APPARATUS:
500ml Beaker, stirrer, 1000ml volumetric flask
REAGENT:
Distilled water, CuSO4 powder
PROCEDURE:
1. Weigh
69.3g of CuSO4 powder into a 500ml beaker, add 200ml distilled water and stir
to dissolve
2. Transfer
to 1000ml volumetric flask, make up to the mark with distilled water and shake
to homogenise
Preparation of Fehling II solution
REAGENT:
Distilled water, NaOH pellets, Potassium sodium tartarate powder
PROCEDURE:
1. Weigh
100g of NaOH pellets into a 500ml beaker, add 200ml distilled water and stir to
dissolve
2. Weigh
346g of potassium sodium tartarate into another 500ml beaker, add 300ml distilled
water and stir to dissolve
3. Transfer
both solutions to 1000ml volumetric flask, make up to the mark with distilled
water and shake
NB:
The above preparations are for the preparation of 1 litre of the resulting
solution.
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