HOW TO DETERMINE PHOSPHATE IN FOOD (VANADOMOLYBDATE METHOD)

DETERMINATION OF PHOSPHATE IN FOOD (VANADOMOLYBDATE METHOD)

AIM:
To determine phosphate content in a food sample.

APPARATUS:
Test tubes, colorimeter.

REAGENT:
Ammonium molybdate, ammonium vanadate, nitric acid.

PRINCIPLE:
Phosphate reacts with vanadomolybdate reagent to form a yellow complex.

PROCEDURE:

• Prepare sample extract.

• Add reagent and incubate for 10 minutes.

• Measure absorbance at 400–420 nm.

CALCULATION:
Compare with phosphate standard curve.