How To Prepare Liebermann’s Reagent - BIOSCIENCE EDUCATION

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Friday, 19 June 2026

How To Prepare Liebermann’s Reagent

 

Liebermann’s Reagent

Purpose: Colorimetric spot test for alkaloids, especially morphine and codeine.

Procedure:

  1. Slowly add 1 ml concentrated sulfuric acid to 1 ml of cold fuming nitric acid in a chilled container.

  2. Mix well and use immediately.

⚠️ Use extreme caution due to strong acids and fumes.

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